Movie of Particle Movement: (See Figure 4 for movement analysis.)

This four minute, time-lapse movie (clock in seconds) depicts the movement of a particle, detected by a GFP-MS2 chimera bound to an ASH1 reporter, in budding yeast cells (mother on bottom, daughter on top). While the particle in the wildtype yeast strain (left) travels through the bud neck and localizes to the bud tip, the particle in the yeast strain containing a deletion of she1 (right) exhibits minimal displacement and does not leave the mother cell. In the wildtype strain, the particle's peak velocity reaches 440 nm/s. (bar = 2 micrometers)